mouse antihuman cd138  (Bio-Rad)

Journal: Experimental Biology and Medicine

Article Title: PEGylated long-circulating liposomes deliver homoharringtonine to suppress multiple myeloma cancer stem cells

doi: 10.1177/1535370216685008

Figure Lengend Snippet: Inhibitive effects of LCL-HHT-H-PEG on MM CD138−CD34−−−CSCs in vitro. (a) Proliferative inhibition assay. (b) Clone formation rate of MM CD138−CD34−CSCs. (c) Quantification of clone formation rate. (d) MM CSC apoptosis, G1 and S phases in cell cycle analyzed by FCM as described in the section of Materials and methods. (e) Quantification analysis of percentage of MM CSC cycle. *P < 0.05, **P < 0.01, and ***P < 0.005. (A color version of this figure is available in the online journal.)

Article Snippet: 20 – 22 Preparation of CD138 − CD34 − MM CSCs CD138 − cells were isolated from human MM RPMI 8226 cells using mouse antihuman CD138 monoclonal antibody coupled to magnetic microbeads (Miltenyi Biotec, Germany) followed by magnetic column selection, using immune magnetic activated cell sorting (Miltenyi Biotec, Germany).

Techniques: In Vitro, Inhibition

Journal: Experimental Biology and Medicine

Article Title: PEGylated long-circulating liposomes deliver homoharringtonine to suppress multiple myeloma cancer stem cells

doi: 10.1177/1535370216685008

Figure Lengend Snippet: Therapeutic effects of LCL-HHT-H-PEG on MM CD138−CD34−−−CSCs in vivo. (a) Dynamic curves of NOD/SCID weight. (b) Images of MM-bearing mice injected with MM CD138−CD34−CSCs on day 30. (c) Images of tumor sizes after the mice were treated with the HHT or LCL-HHT-H-PEG formulation once three-day with total of 10 times as described in the section of Materials and methods. (d) Quantification of tumor sizes. (e) MM-bearing mice’s survival. *P < 0.05 and **P < 0.01. (A color version of this figure is available in the online journal.)

Article Snippet: 20 – 22 Preparation of CD138 − CD34 − MM CSCs CD138 − cells were isolated from human MM RPMI 8226 cells using mouse antihuman CD138 monoclonal antibody coupled to magnetic microbeads (Miltenyi Biotec, Germany) followed by magnetic column selection, using immune magnetic activated cell sorting (Miltenyi Biotec, Germany).

Techniques: In Vivo, Injection, Formulation

Journal: Experimental Biology and Medicine

Article Title: PEGylated long-circulating liposomes deliver homoharringtonine to suppress multiple myeloma cancer stem cells

doi: 10.1177/1535370216685008

Figure Lengend Snippet: Bone mineral density was increased in MM CD138−CD34−CSC xenograft mice treated with LCL-HHT-H-PEG formulation. (a) Bone mineral density in normal mouse and model mouse injected with MM CD138−CD34−CSCs scaned by in vivo Micro-CT on Day 30. (b) Quantification of whole mouse bone mineral density. (c) A representative clinical images of NOD/SCID mice treated with the HHT or LCL-HHT-H-PEG formulation once three-day with total of 10 times. (d) Quantification of whole mouse bone mineral density. *P < 0.05, **P < 0.01, and ***P < 0.005

Article Snippet: 20 – 22 Preparation of CD138 − CD34 − MM CSCs CD138 − cells were isolated from human MM RPMI 8226 cells using mouse antihuman CD138 monoclonal antibody coupled to magnetic microbeads (Miltenyi Biotec, Germany) followed by magnetic column selection, using immune magnetic activated cell sorting (Miltenyi Biotec, Germany).

Techniques: Formulation, Injection, In Vivo, Micro-CT